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A Potential Complete Cure for Cancer:

As a researcher, in my youth at UCLA, I found that the only thing that prevented my rats from getting a fibrosarcoma after giving them a challenge dose of 10 million cells was the antibody I made in sharks. I also gave this antibody to rats that had growing tumors and it significantly reduced the growth rate and shrunk the tumor.

I attribute this phenomena to the fact that sharks only make only one class of antibodies called IgM. This type of antibody was one of the first antibodies to evelove. Sharks have not been observed to contract cancers. This may be due to the fact that IgM is a very primitive antibody and that cancer cells can be thought of as primitive cells. Shark IgM is very efficient at binding serum Compliment.

Compliment is something we all have circulating in our blood, and is part of our immune system. Compliment is a whole set of proteins designed to drill holes into cells and bust them. Compliment will work on any type of cell provided you can activate the compliment to start building its little drill on top of the cell. I have observed that shark compliment and coagulation factors are activated by rat and bovine serum components. All of these serum components appear to be interchangeable among vertebrates.

Here's a cookbook method as to how the shark work has to be done:

Have your patient's tumor removed and place ¼ of it into a tissue grinder with suitable human growth media, add DMSO and glycerol to it prior to freezing it in liquid Nitrogen for future reference. Do not let them place your entire patient's tumor in formalin for biopsy, (that would destroy their tumor cell surface specific antigens).

The other ¼ of the tumor tissue specimen should be placed into tissue culture media, Eagle's MEM or RPMI 1640 with 10-20% BSA will do. If it possible to grow large quantities of the tumor in tissue culture for injection into sharks for antibody production.

I would take the remaining ½ and divide it up into 20 parts after placing it into a sterile tissue homogenizer to make a cell suspension out of it. These parts several should be placed in a -70 freezer for storage, as well, but these are going to be what is used to repeatedly inoculate the shark to produce antibody to your patient's tumor.

To make the shark antibody (anti-serum), take several (4-5) of the most common 3 different species of easily managed 3-4 foot sharks. Inject the aliquot after mixing it in "Complete Freund's Adjuvant." This will stimulate the sharks into making lots of antibody, (the best place to inoculate the sharks is to inject the shark where the lateral fins meet the body dorsally). Wait one week and inject again, do this for a month. Large tropical sharks in warm water might do well for this, they may generate large quantities of antibody faster.

Once you have made the antibodies in the sharks, you will need to bleed the sharks for serum, this is done by using a compound known as FinQuel in a small tank which will anesthetize the sharks. Blood is best drawn for directly below where the tail starts, mid-ventrally.

Once you have the blood from the shark, it should be spun down in a Heparin containing tube to prevent clotting. The blood cells should be placed in suitable growth media. Since all the shark blood cells are nucleated, these cells can replicate in tissue culture using standard methodology. It would be worth trying to see if a set of tumor specific monoclonal antibodies can be made from some of the nucleated shark white cells by genetic engineering into a mouse-human antibody producing hybridoma-fusion strain. Companies such as, Chemicon, in Temecula, CA, have expertise in such antibody production methods.

The remaining plasma contains antibodies to your patient's normal white and red blood cells, but also contains active shark Compliment cell lysis factors. The plasma must be heated to 56 C for 30 minutes in a water bath. This will denature the shark compliment, leaving a whole set of different active antibodies. Some of the antibody's will be formed to any of the normal white and red blood cells, and normal tissue components that we injected into the shark at the original tissue used for inoculation. That antibody to normal tissue components has to be absorbed against your patient's normal washed white and red blood cells. Performed by adding your patient's red and white cells to the shark serum for a few hours in the refrigerator. Discarding the resultant absorbed cells by centrifuge keeping the supernatant containing the antibodies to your patient's specific tumor antigens.

You are now ready to inject the antibodies IV. If you followed the above procedure you will have made shark antibodies against your patient's tumor cells and you should inject your patient with these antibodies daily for several weeks. I would use small doses at first and gradually increase the dose. Do not stop until you run out of shark antibody.

If your patient begins getting serum sickness you will have to change serum and use one of the different species of shark you have been immunizing. Hopefully the sharks were selected from unrelated groups so the patient's antibodies to the shark serum antibodies will not cross react. By daily injection of your patient serum sickness may be avoided by the mechanism of induced low to high dose tolerance. By the time the patient starts to get serum sickness, the monoclonal hybridoma produced humanized antibodies can then be substituted. (If it was possible to generate them).

To determine if your shark tumor therapy has been effective, it is possible to use the tumor specific antibodies generated in the shark, to be linked to a fluorescent, or luminescent luciferase marker. At the time of the mouse-hybridoma procedure, a hybridoma-luciferase or hybridoma-fluorescent antibody-protein marker can be attached. I refer you to www.prolume.com BioLight Diagnostics company division.

Much of this decision making would depend on the type of tumor, and where it was located.

I have personally tried the above serum making procedure (but not the monoclonal hybridoma fusions) and the results indicated that a complete cure could be obtained for even very large tumors in rats. For humans, it would be advisable to de-bulk as much tumor as possible surgically prior to the serum injections. Once compliment factors are initiated, large amounts of intracellular potassium and active cell substances are released rapidly into the bloodstream. Patients would have to be monitored for electrolyte and respiratory distress that could occur if large amounts of cell lysis products are introduced into the patient's blood stream rapidly.

If you need additional information or wish to dispute what I say here please feel free to call or write me.

Thank you for reading this.

Bruce Bryan, MD

PS: This method will be un-patentable one year from this public disclosure, June 1, 2000